- Aunt, by marriage, of singer Aaliyah (she was married to Aaliyah's uncle Barry Hankerson).
- Inducted into the Rock and Roll Hall of Fame (as a member of Gladys Knight and the Pips) in 1996.
- She has three children, sixteen grandchildren and six great-grandchildren.
- The name, "The Pips", came from the name of their manager, James "Pip" Woods, who is also Knight's cousin.
- She owns a chain of chicken and waffles restaurants based in Atlanta.
- She was awarded a Star on the Hollywood Walk of Fame for Recording at 7083 Hollywood Boulevard in Hollywood, California on June 27, 1995.
- Ranked #18 on VH1's 100 Greatest Women of Rock N Roll
- Is a member of Alpha Kappa Alpha Sorority, Inc.
- Mother of Jimmy Newman III (died 1999), Kenya Jackson (née Newman), and Shanga Hankerson.
- Following the death of her friend Aretha Franklin due to pancreatic cancer in August 2018, she admitted that she is a stage I breast cancer survivor. Due to early detection, she is cancer-free now.
- Won on Ted Mack & the Original Amateur Hour (1948) at age seven.
- Has an older sister, Brenda Knight, and a younger brother, David Knight.
- Currently tours with her choir "Saints Unified Voices" throughout the United States at LDS (Mormon) church meetinghouses, cultural events and concerts. Ray Charles' last album contains a duet with Gladys Knight which received a Grammy for Best Gospel Performance (Duet). (February 2005)
- Parents are Merald Woodlow Sr. and Sarah Elizabeth Knight.
- Once performed "Licence to Kill" in concert at Caesars Atlantic City for Jason Allentoff and Brandon Allentoff.
- Had a gambling problem.
- For many years, she used to watch The Hour of Power (1970) that featured Robert H. Schuller. She made a guest-appearance on the show, decades later.
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