The movie was filmed in Maryland, which is why greenery is in evidence despite the post-apocalyptic setting. The large building into which the murderous deacons chase Sam Asgarde was a disused tobacco curing facility.
Screenwriter Tim Sullivan loved science fiction and horror movies when he was growing up. The cow that is central to the plot of this movie is named Gertrude, named after the duck in Journey to the Center of the Earth (1959), one of his favorite films.
In some scenes, Sullivan as Sam Asgarde bares his teeth as he faces the enemy and/or fires his weapon. Consultant Walter Suarez, who was a Vietnam veteran, advised Sullivan not to show his teeth, because soldiers use camouflaging face paint and mud for a reason: to be inconspicuous. A soldier should never show bared teeth, their whiteness can make him a target. Sullivan decided to go for the drama rather than the realism.
The trained dog Tex, who appears in the opening minutes of the movie, also "acted" in Sommersby (1993) starring Jodie Foster and Richard Gere. Writer/star Tim Sullivan joked later, "The dog was the most famous member of our cast."
The film has never been released (although pirate copies of the promotional 1997 VHS "screener" tape have been bootlegged over the years. It has never been seen under it's original title of TWILIGHT OF THE DOGS. A distributor in 1997 changed the title to "New Genesis: Twilight of the Dogs" but were never able to sell it.