Sailor V appeared in this one, in Japanese, as a non-speaking role. She was officially the first Sailor Scout ever featured.
In the Manga Motoki / Andrew is introduced in this one, however he is not in the Anime.
In the anime Queen Beryl appears in this one, but doesn't until the second in the Manga.
Some scenes were cut from the Japanese Dub. They included the follow: Serena eating in the hallway, Serena kicking the door when Sammy locks her out, the opening scene in which Serena talks about herself and her destiny.
Major Content changes happened between Japanese Dub and English Dub. The English dub changed the scene in which Melvin scolded Serena for her test score and told her about Sailor V to Melvin asking about her test and Molly inviting her to go shopping at a sale.