The anime characters named Kichiro and Mojiro who guide Stan through the process are based on the mascots from the 2005 Aichi World Fair.
Principal Lewis says to tell Esteban "el lobo negrón" says hello. This means "the black wolf" in Spanish.
During Stan's post-vasectomy sex, he mentions a position called the "Reverse Bulldog" to Francine, adding that she needs to punch him harder. The Reverse Bulldog is commonly known as a wrestling move.
Roger (Seth MacFarlane) sings "Moonlight Becomes You" from 'Road to Morrocco.' On Seth MacFarlane's other series, 'Family Guy,' Brian and Stewie sang a parody of this film's title song called "Road To Rhode Island."