As Wasp is in the 2012 Avengers, it means Hank trained Hope as Janet's successor.
As Hulk isn't in the Battle of New York, it means Peggy didn't have her blood drawn to isolate samples of Erskine's serum, like they did for Steve in the Sacred Timeline. So there was no way for Bruce to accidentally turn himself into the Hulk; he didn't have a formula or poorly recreated sample.
However, it was shown in S1 that blood samples were being taken from her. Also there's Wasp during the NY Battle, who was absent in Sacred Timeline (if it's Pym's wife) or didn't take the suit yet (if it's Hope).
This episode combines the plots of Cap 2: Winter Soldier and Black Widow. Befitting as the Red Room is Russian in origin.
Unlike Steve, who struggled with the 21st century for a time, Peggy adjusts very quickly and enjoys the freedom women have and the new technology.
Peggy and Natasha are good friends by the Battle of New York, implying Peggy didn't spend time in a depression like Steve did, and started working for SHIELD off the bat.