Optimus Prime's trailer has markings on its side that resemble the iconic stripes of the original Optimus' trailer, from the eighties.
The train the Autobots, Fowler and the military use to escort the D.N.G.S. is modeled after a real-life train assembled by Electro-Motive Diesel.
On board the train, Jack grabs a fire extinguisher, while Miko attempts to fight with an axe. Both of these items have been their respective weapons of choice in previous episodes.
The original Takara toy that would eventually become Optimus Prime in 1984 was named Convoy, sharing a name with this episode. Current Optimus Prime figures distributed by Takara, such as the Masterpiece MP-10, are still branded under the same name.