The film originally included more darker elements, a famous one being a deleted sequence from the kitchen scene. In this sequence the Duke bakes a live baby skunk in a pie and then eats it. Oddly enough, according to Gary Goldman, the reason this was cut wasn't because of the content but because Bluth's studio received a complaint from Goldcrest's marketing representative that most cases of child abuse happen in the kitchen, and involve baking instruments.
The final scene's blending live action with animation was inspired by the popularity of it in Who Framed Roger Rabbit (1988). Similarly, Goldie the pheasant's sultry appearance was slightly based off of that of Jessica Rabbit. As shown in one of the early trailers (found on the original 1990 VHS release of All Dogs Go to Heaven (1989)), Goldie was also originally given large cleavage, identical to that of Jessica Rabbit. However, after mothers at test screenings were scandalized by her outrageous proportions, Bluth's animators were ordered by Goldcrest Films to censor Goldie's cleavage.
Glen Campbell was really good friends with Elvis Presley and would sometimes do Elvis imitations in his concerts. He stopped doing this once Elvis died. For this film, he once again bases his singing voice on Elvis.
Pinky (The Fox), the sinister and greedy manager of Chanticleer (The King) is based on the late Elvis Presley's band manager Colonel Tom Parker.